Healthcare workers & SARS-CoV-2 infection in India: A case-control investigation in the time of COVID-19

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Indian J Med Res, Epub ahead of print DOI: 10.4103/ijmr.IJMR_2234_20

Pranab Chatterjee1,#, Tanu Anand7,#, Kh. Jitenkumar Singh2, Reeta Rasaily3, Ravinder Singh4, Santasabuj Das8, Harpreet Singh5, Ira Praharaj6, Raman R. Gangakhedkar6, Balram Bhargava† & Samiran Panda9

1Translational Global Health Policy Research Cell, †Department of Health Research, Ministry of Health & Family Welfare, 2ICMR-National Institute of Medical Statistics, 3Division of Reproductive Biology, Maternal Health & Child Health, 4Division of Non-Communicable Diseases, 5Informatics, Systems & Research Management Cell, 6Division of Epidemiology & Communicable Diseases, 7Multidisciplinary Research Unit/Model Rural Health Research Unit, †Indian Council of Medical Research, New Delhi, 8Division of Clinical Medicine, ICMR-National Institute of Cholera & Enteric Diseases, Kolkata,

West Bengal & 9ICMR-National AIDS Research Institute, Pune, Maharashtra, India

Received May 28, 2020

Background & objectives: Healthcare workers (HCWs) are at an elevated risk of contracting COVID-19. While intense occupational exposure associated with aerosol-generating procedures underlines the necessity of using personal protective equipment (PPE) by HCWs, high-transmission e ciency of the causative agent [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] could also lead to infections beyond such settings. Hydroxychloroquine (HCQ), a repurposed antimalarial drug, was empirically recommended as prophylaxis by the National COVID-19 Task Force in India to cover such added risk. Against this background, the current investigation was carried out to identify the factors associated with SARS-CoV-2 infection among HCWs in the country.

Methods: A case-control design was adopted and participants were randomly drawn from the countrywide COVID-19 testing data portal maintained by the ICMR. The test results and contact details of HCWs, diagnosed as positive (cases) or negative (controls) for SARS-CoV-2 using real-time reverse transcription-polymerase chain reaction (qRT-PCR), were available from this database. A 20-item brief-questionnaire elicited information on place of work, procedures conducted and use of PPE.

Results: Compared to controls, cases were slightly older (34.7 vs. 33.5 yr) and had more males (58 vs. 50%). In multivariate analyses, HCWs performing endotracheal intubation had higher odds of being SARS-CoV-2 infected [adjusted odds ratio (AOR): 4.33, 95% con dence interval (CI): 1.16-16.07]. Consumption of four or more maintenance doses of HCQ was associated with a signi cant decline in the odds of getting infected (AOR: 0.44; 95% CI: 0.22-0.88); a dose-response relationship existed between frequency of exposure to HCQ and such reductions (χ2 for trend=48.88; P<0.001). In addition, the use of PPE was independently associated with the reduction in odds of getting infected with SARS-CoV-2.

#Equal contribution

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Interpretations & conclusions: Until results of clinical trials for HCQ prophylaxis become available, this study provides actionable information for policymakers to protect HCWs at the forefront of COVID-19 response. The public health message of sustained intake of HCQ prophylaxis as well as appropriate PPE use need to be considered in conjunction with risk homoeostasis operating at individual levels.

Key words Dose-response relationship – healthcare worker – hydroxychloroquine prophylaxis – personal risk management – rapid evidence generation – SARS-CoV-2

Since its global recognition in December 2019, the novel coronavirus disease (COVID-19) pandemic has spread to over 200 countries in less than ve months. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of this disease, was noted to spread e ciently through respiratory droplets and contact routes1-4. While common presenting symptoms are fever, fatigue, dry cough, myalgia and dyspnoea, a few patients have reported having diarrhoea, nausea, vomiting and new-onset anosmia or ageusia. A considerable proportion of the SARS- CoV-2-infected individuals (around 80%) did not have any noticeable symptoms and yet were able to transmit the infection5. Such unique transmission potentials of SARS-CoV-2 and lack of de nitive antiviral therapy were the reasons behind its wide-scale spread. Evidence indicates that healthcare workers (HCWs) are particularly at risk of acquiring SARS-CoV-2 infection, due to repeated occupational exposure6.

In the absence of speci c treatments against COVID-19, social distancing7, use of face masks8 and frequent hand washing with alcohol rubs or soap constituted the infection prevention measures targeting general population9. However, HCWs, being exposed to a higher quantum of risk, needed additional intervention approaches for protection10. Aprons, gowns, gloves, masks, face shields and goggles addressed such needs. These protective gears serve useful purpose in settings where procedures such as nasopharyngeal swab collection, endotracheal intubation or respiratory suctioning are performed on suspected or con rmed patients of COVID-19, potentially generating aerosols from the respiratory tract11. However, caregiving in a pandemic situation would also entail the risks of transmission of SARS-CoV-2 infection to HCWs from asymptomatic individuals who are not necessarily undergoing invasive procedures2,12,13. Chemoprophylaxis for HCWs could potentially have add-on advantages to cover this additional risk.

Prophylaxis in the present context refers to the use of a short-term therapy to prevent acquisition

of SARS-CoV-2 infection. Currently, there are no approved vaccines against SARS-CoV-2, which makes the alternative of using chemotherapeutic agents an attractive proposition. However, no antiviral medicines proved e cacious during the previous coronavirus outbreaks (SARS 2003; Middle East respiratory syndrome coronavirus 2012) and therefore, did not leave the therapeutic community with any viable options during the present COVID-19 pandemic14,15. Hydroxychloroquine (HCQ) came into discussion against this background16. Ability of this compound to inhibit the infection by SARS-CoV-2, as well as viral replication in cell cultures in a time- and dose-dependent manner made it a primary choice17. Furthermore, HCQ elevates the pH of endosomes and inhibits SARS- CoV-2 RNA-mediated in ammatory response18. These laboratory ndings encouraged researchers to consider HCQ, originally used for malaria, as a repurposed agent for prophylaxis against SARS-CoV-219.

The National Task Force for COVID-19 in India took cognizance of this evidence and empirically recommended the use of HCQ as prophylaxis against SARS-CoV-2 infection in asymptomatic HCWs treating suspected or con rmed COVID-19 cases. Asymptomatic household contacts of con rmed COVID-19 cases were also covered by this advisory released on March 22, 202020. Around the same time, in South Korea, HCQ prophylaxis was used successfully to avert new infections after a large COVID-19 exposure event in a long-term care facility21. Scienti c communications further underlined the necessity of examining the utility of such approaches in the context of high-burden, high-income countries such as Italy22. Against this backdrop, a case-control investigation was conducted to compare the risks of and protective factors against SARS-CoV-2 infection among HCWs in India.

Material & Methods

The ICMR COVID-19 Research Team developed the study proposal, which was approved by the ICMR

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Central Ethics Committee. Data collection for this investigation was done during May 8-23, 2020. Each participant was informed about the study purpose, and verbal consent was obtained before proceeding with telephonic interview. A data portal developed to capture the information regarding individuals undergoing testing for SARS-CoV-2 infection across India was used to identify the study participants. HCWs tested between the rst week of April 2020 and the end of rst week of May 2020 formed the sample pool, from which cases and controls were drawn. Symptomatic HCWs testing positive on real-time reverse transcription-polymerase chain reaction (qRT-PCR) for SARS-CoV-2 were de ned as cases. Controls were symptomatic HCWs who tested negative on qRT-PCR for SARS-CoV-2 under similar considerations.

Measures: A brief 20-item interview schedule was developed to elicit the information on key issues, such as department, designation and length of employment, and use of personal protective equipment (PPE). Among exposure variables, the HCW was asked about contact with suspected or con rmed COVID-19 patients on ventilator and involvement in aerosol-generating procedures (AGPs) such as nasopharyngeal swab collection, endotracheal intubation and respiratory suction. To minimize recall bias, this enquiry was restricted to seven days before SARS-CoV-2 testing. A history of prophylactic HCQ intake with dosing details was also obtained.

Telephonic interviews: Participants were telephonically contacted by the researchers to introduce themselves, verify identities, describe the study purpose and check availability for interviews. If a participant’s contact phone number in the ICMR data portal actually belonged to a treatment supporter or caregiver or relative, we reached out to the individual who was tested for COVID-19 through the primary contact. Following verbal consent, telephonic interviews, which took 5-11 min, were conducted. At the close of the interviews, participants’ queries related to COVID-19 were addressed.

Sample size: It was intended to enrol cases and controls in a 1:1 ratio and match them for location (testing centre) and temporality (test date). Assuming that 50 per cent of the controls were on HCQ prophylaxis (exposure) and correlation coe cient for exposure between matched cases and controls would be 0.2, it was estimated that 484 cases would be required to detect an odds ratio of 1.50 with 80 per cent power at ve

per cent signi cance level23. These calculations were undertaken using Power Analysis Sample Size (PASS) software version 11.024.

Statistical analysis: The data captured in hard copies during the telephonic interviews were checked for quality and computerized following the necessary corrections. The association of key risk factors with SARS-CoV-2 infection was examined by comparing distributions of cases and controls across di erent exposures. Variables which had biologically plausible association with the outcome and were relevant for planning strategies for the prevention of SARS-CoV-2 infection in HCWs were entered into a standard logistic regression model25. STATA version 13.1 (StataCorp LP, College Station, TX, USA) was used for data analysis including trend analysis by Chi-square test.

Results

The ICMR data portal contained the results and contact details of 23,898 symptomatic HCWs who were tested for SARS-CoV-2 infection. After excluding non-Indian nationals and missing or wrong contact details from this database, 21,402 records were obtained, with 1,073 (5%) con rmed SARS- CoV-2-infected HCWs. Although it was initially decided to contact 650 cases and controls each (accounting for 25% loss over the calculated sample size of 484), only 624 and 549 individuals could be contacted in the case and control groups, respectively. Completed interview schedules of 60.58 per cent of cases (378/624) and 67.94 per cent of controls (373/549) were available for analysis. The reasons for not being able to reach out to some of the participants were: calls not picked up, wrong numbers, ineligible candidates (not HCWs), consent refusal to name a few.

Fifty eight per cent of the cases and about half of the controls were males. While the mean age of the cases was 34.73 yr [±standard deviation (SD): 9.64; median: 33.0; interquartile range (IQR): 27-40], the mean age of the controls was 33.47 yr (±SD: 9.77; median: 31.0; IQR: 26-38). Age distribution did not follow Gaussian distribution in either group. Table I presents details of the study participants.

Vulnerability of HCWs: Vulnerability of the study participants to SARS-CoV-2 infection was ascertained through a history of (i) placement in intensive care unit (ICU) catering to suspected or con rmed COVID-19 cases, (ii) procedures such as nasopharyngeal swab collection, intubation, respiratory suctioning and

CHATTERJEE et al: HCQ PROPHYLAXIS IN HCW IN INDIA 3

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Table I. Pro le of the healthcare workers included in the study

Parameters Cases (n1=378) (%) Controls (n2=373) (%) OR 95% CI of OR P

Gender

Male 219 (57.95) 188 (50.40) 1.36 1.02-1.81 0.038 Female 159 (42.06) 185 (49.60) Ref

Age (yr)

18-25 26-33 34-41 42-49 >50

65 (17.2) 134 (35.45) 97 (25.66) 43 (11.38) 39 (10.32)

86 (23.06) 135 (36.19) 77 (20.64) 43 (11.53)

0.62 0.35-1.09 0.208 0.81 0.48-1.38
1.04 0.59-1.8
0.82 0.44-1.54

32 (8.58) Ref

Occupation

Doctor
Nurse/ANM
Housekeeping sta
Security guards
Others
Laboratory technician/operation theater technician

111 (29.37) 165 (43.65) 16 (4.23) 10 (2.65) 36 (9.52) 40 (10.58)

123 (32.98) 144 (38.61) 10 (2.68) 12 (3.22) 42 (11.26)

0.94 0.57-1.57 1.2 0.74-1.96 1.68 0.68-4.14 0.88 0.34-2.25 0.9 0.48-1.67

0.537

42 (11.26) Ref

Duration of work in respective workplace before COVID-19 testing (yr)

>1 264 (69.84) 179 (47.99) 2.51 1.86-3.39 <0.001

<1 114 (30.16) 194 (52.01) Ref

OR, odds ratio; CI, con dence interval; Ref, reference category; ANM, auxiliary nurse midwife

clinical specimen handling by HCWs and (iii) use of PPE. Endotracheal intubation was associated with higher odds of SARS-CoV-2 infection. Respondents who reported never using PPEs were also at a higher risk. On the other hand, when the participants were asked about individual components of PPE, usage of masks, caps, gowns and gloves was associated with reduced odds of acquiring SARS-CoV-2 infection (Table II).

Hydroxychloroquine prophylaxis: Distribution of cases and controls across exposures in univariate analysis indicated the association of risk (P=0.087) of SARS-CoV-2 infection with the lack of HCQ prophylaxis (Table III). However, the number of maintenance doses taken by HCWs following the intake of a loading dose revealed a protective dose- response relationship. Consumption of four or more maintenance doses was associated with a signi cant decline in the risk of SARS-CoV-2 infection among the study participants (Figure). The signi cant declining trend had an overall χ2 value of 48.88 (P<0.001).

70 60 50 40 30 20 10

Trend line
Graph with data points

No HCQ

HCQ without maintenance

HCQ with 2-3 doses

HCQ with 4-5 doses

HCQ with 6 & above doses

Exposure intensity to HCQ

Figure. Dose-response relationship between hydroxychloroquine (HCQ) exposure and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

Of the 172 cases and 193 controls reporting HCQ intake, no signi cant di erence in the occurrence of adverse drug reactions was noted. The three most common side e ects of HCQ as reported by the cases and controls were nausea (5 vs. 8%), headache (6 vs. 5%)

Proportion positive for SARS-CoV-2 (%)

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CHATTERJEE et al: HCQ PROPHYLAXIS IN HCW IN INDIA Table II. Place of work, procedure and protection details of healthcare workers

0.17 0.488 0.01 0.365

0.603 <0.001

<0.001 0.018 0.001 0.743 0.158 <0.001

Yes
No
PPE usage
Never used
Used in all or some cases
Use of PPE gears
Masks
Any mask use
No mask use
Cap
Yes
No
Gown
Yes
No
Shoe cover use
Yes
No
Face shield or goggles
Yes (either or both)
No (none)
Gloves
Yes
No
PPE, personal protective equipment; ICU, intensive care unit

Cases (n1=378) (%)

53 (14.02) 325 (85.98)

18 (4.76) 360 (95.24)

22 (5.82) 356 (94.18)

15 (3.97) 363 (96.03)

Controls (n2=373) (%)

OR 95% CI of OR

ICU with suspected or con rmed COVID-19 cases on ventilator

Yes

Nasopharyngeal swab collection

Yes

Endotracheal intubation

Yes

Respiratory tract suctioning

Yes

Handling clinical specimen (stool, blood, bronchoalveolar lavage)

1.36 0.88-2.1

42 (11.11) 336 (88.89)

57 (15.08) 321 (84.92)

310 (82.01) 68 (17.99)

166 (43.92) 212 (56.08)

152 (40.21) 226 (59.79)

133 (35.19) 245 (64.81)

163 (43.12) 215 (56.88)

267 (70.63) 111 (29.37)

46 (12.33)
327 (87.67) Ref

40 (10.72)
333 (89.28) Ref

22 (5.9)
351 (94.1) Ref

9 (2.41)
364 (97.59) Ref

20 (5.36)
353 (94.64) Ref

17 (4.56)
356 (95.44) Ref

346 (92.76)
27 (7.24) Ref

196 (47.45)
177 (52.55) Ref

194 (52.01)
179 (47.99) Ref

127 (34.05)
246 (65.95) Ref

180 (48.26)
193 (51.74) Ref

322 (86.33)
51 (13.67) Ref

0.8 0.42-1.51

2.5 1.13-5.5

0.73 0.37-1.45

0.89 0.57-1.39

3.72 2.12-6.52

0.35 0.22-0.57

0.7 0.53-0.94

0.62 0.46-0.83

1.05 0.78-1.42

0.81 0.61-1.08

0.38 0.26-0.55

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Table III. Patterns of hydroxychloroquine (HCQ) prophylaxis in healthcare workers

Parameters

Cases (n1=378) (%)

Controls (n2=373) (%)

95% CI of OR

HCQ prophylaxis

206 (54.5)

180 (48.26)

1.28

0.96-1.71

0.087

Yes

172 (45.50)

193 (51.74)

Ref

Number of maintenance doses of HCQ prophylaxis taken

12 (3.17)

56 (15.01)

0.19

0.1-0.36

<0.001

4-5

42 (11.11)

67 (17.96)

0.55

0.35-0.84

2-3

70 (18.52)

37 (9.92)

1.65

1.06-2.58

HCQ loading dose and irregular recall of maintenance

48 (12.7)

33 (8.85)

1.27

0.78-2.07

None

206 (54.5)

180 (48.26)

Ref

Combination prophylaxis

HCQ only

130 (34.39)

133 (35.66)

0.85

0.62-1.17

0.002

HCQ+azithromycin+vitamins

25 (6.61)

16 (4.29)

1.36

0.71-2.64

HCQ+vitamins

6 (1.59)

25 (6.70)

0.21

0.08-0.52

HCQ+non-allopathic systems of medicines or others

11 (2.91)

19 (5.09)

0.51

0.23-1.09

No HCQ

206 (54.5)

180 (48.26)

Ref

and diarrhoea (5 vs. 4%). While none of the controls on HCQ complained of palpitations, only one case (1/172, 0.6%) reported the same. Gastrointestinal symptoms such as acidity and vomiting following HCQ intake ranged from 0.6 per cent in cases to about two per cent in controls. Very few cases (0.6%) and controls (1.4%) had skin rashes after consuming HCQ.

Multivariate analysis: Factors found associated (P<0.1) with SARS-CoV-2 infection among HCWs in univariate analysis and having biological plausibility were entered into multivariate model. In case of conceivable similarity between explanatory variables, one was chosen over another to avoid collinearity. For example, PPE rather than individual items (cap, mask, gown, glove, etc.) of PPE was included in the model. Adjusted for25 gender, use of PPE, endotracheal intubation, di erent intensity of exposure to prophylactic HCQ and testing place with date, intake of 4-5 maintenance doses of HCQ [adjusted odds ratio (AOR): 0.44; 95% con dence interval (CI): 0.22- 0.88; P=0.02] was found to independently impart the protective e ect against SARS-CoV-2 infection among HCWs (Table IV). Notwithstanding this e ect, the advantage of PPE usage was also independently indicated by the multivariate model. Noticeably, six or more prophylactic doses of HCQ used by HCWs had a remarkably high (>80%) protective e ect against SARS-CoV-2 infection.

Discussion

Research to inform public health responses during infectious disease emergencies is gradually gaining importance worldwide. For example, Ebola virus disease in West Africa and Nipah virus outbreak in the Indian sub-continent required quick research responses to help mitigate human su erings in the recent past26,27. The current investigation can be considered as an example of this emerging trend. We leveraged a nationwide COVID-19 testing database to rapidly generate evidence to inform public health action.

The pivotal nding of our study was the noteworthy bene ts of HCQ prophylaxis. It was identi ed that simply initiating HCQ prophylaxis did not reduce the odds of acquiring SARS-CoV-2 infection among HCWs. However, with the intake of four or more maintenance doses of HCQ, the protective e ect started emerging, and in the adjusted multivariate model, a signi cant reduction (>80%) in the odds of SARS-CoV-2 infection in the HCWs was identi ed with the intake of six or more doses of HCQ prophylaxis. This dose-response relationship (Figure) added strength to the study outcomes. Worth noting in this context was that the National Task Force for COVID-19 in India recommended once a week maintenance dose for seven weeks (400 mg once weekly), following the loading dose (400 mg bd).

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CHATTERJEE et al: HCQ PROPHYLAXIS IN HCW IN INDIA 7

Table IV. Factors independently associated with SARS-CoV-2 in healthcare workers in India

Attributes

AOR

95% CI of AOR

Male

1.93

1.21-3.07

0.006

Never used PPE

5.33

2.27-12.48

<0.001

Performing endotracheal intubation

4.33

1.16-16.07

0.029

Maintenance doses of HCQ

HCQ loading dose and irregular recall of maintenance

1.87

0.82-4.24

0.136

2-3

2.34

1.23-4.83

0.022

4-5

0.44

0.22-0.88

0.02

≥6

0.04

0.01-0.16

<0.001

AOR, adjusted odds ratio; HCQ, hydroxychloroquine

Adherence to this recommended regimen is underlined by the ndings of the present study. The potential antiviral and anti-in ammatory properties of HCQ28, together with the low cost of therapy, excellent oral bioavailability29, high tissue concentrations in the lungs relative to the plasma levels and acceptable safety pro le lend support to this assertion17. However, HCQ prophylaxis should be taken in tandem with PPE use as indicated by the multivariate model (Table IV).

A recent registry-based analysis highlighted that HCQ did not o er therapeutic bene ts to severe COVID-19 cases, and was associated with increased mortality30. This apparent disparity with the ndings of the current investigation could be explained by the two di erent application contexts. While the observational study involving registry-analysis focussed on the treatment of hospitalized COVID-19 patients, our emphasis was on the prevention of infections among HCWs. In treatment settings, severe COVID-19 patients are likely to have a very high viral load and cytokine levels, which may not be improved by HCQ therapy31. The registry-based analysis further recorded higher frequencies of ventricular arrhythmias in patients receiving HCQ. The toxicities of HCQ are likely to be infrequent in healthy groups undergoing prophylactic therapy as observed in our study participants. Biologically, it appears plausible that HCQ prophylaxis, before onset of infection, may inhibit the virus from gaining a foothold.

While the strength of the present analysis was the involvement of a countrywide database that drew upon more than 70 COVID-19 testing laboratories spread all over India, its limitations were rooted in its observational design. However, in the absence of clinical trial results32 on safety and e cacy of HCQ chemoprophylaxis in the HCWs, this study

o ers evidence of public health importance. Higher prevalence of SARS-CoV-2 infection in the HCWs has been a global concern, including in countries such as Spain, Italy and the USA33-35, which further underscores the importance of the present ndings.

The rst part of the dose-response relationship curve showed an apparent increase in the odds of acquiring SARS-CoV-2 infection in HCWs who had taken 2-3 doses of HCQ prophylaxis. While this phenomenon cannot be fully explained by the data collected through the present study, lessons from other areas of public health could be of some help. The parallels36 include (i) seat-belt legislations vis-à-vis speeding and road tra c casualties, and (ii) condom use promotion with unintended e ects linked to greater sexual activities. Adams37 and Wilde38 allude to models of individual risk management which have the potential to explain such apparent paradoxes. They described that the introduction of a safety device could disrupt the balance between perceived hazards and rewards of risk-taking behaviours. Within the ambit of the present discussion, we consider (i) HCQ prophylaxis as a newly identi ed safety device, (ii) getting infected with SARS-CoV-2 as the perceived hazard, and (iii) not adhering to conventional respiratory infection prevention measures, such as PPE use, personal hygiene and social distancing as risk-taking behaviours.

In conclusion, public health message on the role of HCQ prophylaxis for the prevention of SARS-CoV-2 infection among HCWs emerging from this study should be considered with the existing understanding of risk homoeostasis operating at individual levels.

Acknowledgment: Authors acknowledge the support in data cleaning and management provided by Servshri Ganesh Prasad Jena, Ajay Kumar and Gurmeet Singh Rana, ICMR-National

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Institute of Medical Statistics (ICMR-NIMS); and in data collection Ms Seema Verma, Division of Non-Communicable Diseases, ICMR, New Delhi.

Financial support & sponsorship: None. Con icts of Interest: None.

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CHATTERJEE et al: HCQ PROPHYLAXIS IN HCW IN INDIA 9

For correspondence: Dr Samiran Panda, ICMR-National AIDS Research Institute, 73-G, MIDC, Bhosari, Post Box No. 1895, Pune 411 026, Maharashtra, India

e-mail: director@nariindia.org